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Which approach could be used to investigate distinctions between a muscle cell and a bone cell?


A) Gene expression profiling
B) DNA variation screening
C) Microarray comparative genomic hybridization
D) Transcription-mediated amplification

E) A) and C)
F) B) and C)

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Genetic modification


A) alters genetic codes so that one species' code is like another's.
B) adds sugars and phosphates in a nucleus.
C) alters,deletes,or adds DNA to a cell.
D) substitutes entire nuclei to genetically engineer a cell.

E) B) and C)
F) A) and B)

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C

To create a transgenic organism,a researcher


A) introduces foreign DNA into a fertilized ovum.
B) injects a gene of interest into a somatic cell.
C) injects a gene of interest into several somatic cells.
D) introduces foreign DNA into somatic cells in culture and transplants them.

E) All of the above
F) C) and D)

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Bacteriophages can be used as vectors in recombinant DNA experiments because they


A) are small and made of double-stranded DNA.
B) are circular and easily imported into bacteria or yeast.
C) insert their genetic material into bacteria.
D) are resistant to protective restriction systems.

E) A) and C)
F) C) and D)

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Tiny fat bubbles used to deliver genes are


A) phospholipids.
B) cholesterols.
C) liposomes.
D) plasmids.

E) None of the above
F) C) and D)

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A multicellular organism that carries DNA from other species is termed


A) transverted.
B) translocated.
C) transgeniC.
D) transformeD.

E) C) and D)
F) B) and C)

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In 1975,scientists convened in Asilomar,California and


A) determined that restriction enzymes could cut DNA.
B) created the first transgenic animals.
C) reviewed the use of drugs produced by recombinant DNA technology.
D) drew up guidelines to regulate recombinant DNA technology.

E) B) and D)
F) C) and D)

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The process that uses short,synthetic,double-stranded RNAs to intentionally bind mRNAs thereby diminishing gene expression is called _____.


A) knockup
B) knock down
C) nonsense
D) bioremediation

E) C) and D)
F) None of the above

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B

A DNA sequence can be patented if


A) two or more people isolate the same sequence successfully.
B) it forms a single gene.
C) it has economic implications.
D) it is useful as a tool for research.

E) None of the above
F) C) and D)

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The first drug produced using recombinant DNA technology was


A) insulin.
B) streptokinase.
C) tissue plasminogen activator.
D) erythropoietin.

E) B) and D)
F) B) and C)

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Natural selection can be used in bioremediation by acting on pre-existing variations among organisms to select those that can detoxify pollutants.

A) True
B) False

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A molecule that consists of a piece of DNA from one organism combined with the DNA from a member of another species is called


A) restricted DNA.
B) recombinant DNA.
C) homogenic DNA.
D) bioengineered DNA.

E) B) and D)
F) B) and C)

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A limitation of transgenesis is that


A) it only works on very short genes.
B) it only works on vertebrates.
C) the transgene can insert many copies of itself in the genome.
D) the transgene could disappear before it inserts in the genome.

E) B) and D)
F) B) and C)

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C

Recombinant DNA technology is used to


A) create many copies of a specific piece of DNA.
B) make more copies of DNA polymerase.
C) copy protein into RNA.
D) make RNA in the cell nucleus.

E) A) and B)
F) A) and C)

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Which of these would not be used to introduce DNA in animal cells?


A) Chemicals
B) Electricity jolts
C) Particle bombardment
D) A Ti plasmid

E) B) and C)
F) A) and D)

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The type of RNA that carries out RNA interference is


A) tRNA.
B) mRNA.
C) rRNA.
D) siRNA.

E) A) and B)
F) A) and C)

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RNA interference was discovered in 1998.

A) True
B) False

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Morpholinos are


A) short DNA sequences found only in populations that trace their roots to Italy.
B) short synthetic molecules similar to DNA that are complementary to splice-site mutations.
C) types of DNA microarrays used to study gene expression.
D) a type of recombinant bacteria.

E) A) and B)
F) A) and C)

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The process in which bacteria with the ability to detoxify certain pollutants are released in a particular area is known as


A) bioremediation.
B) gene silencing.
C) gene expression profiling.
D) enzyme restriction.

E) A) and B)
F) None of the above

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The requirements for patenting of an invention involving DNA in the U.S.are that it should be


A) new,useful,and not obvious to an expert in the field.
B) obvious,and no one should have previously been able to accomplish it.
C) helpful and affordable.
D) new,useful,and predicted by experts in the fielD.

E) A) and D)
F) A) and C)

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